Water Pollution problem in environment are increasing day by day degrading ecosystem and global water cycle. Applications of biological agents, along with physical, chemical & engineering processes to maintain, protect and restore the environment by use of Environmental Biotechnology and analytical analysis and tests like BOD, COD, TSS and TS, etc. are required to assess the pollution load and strength of waste to be treated in most efficient manner. Biological treatment/bioremediation and natural attenuation have been a rapidly growing area of science over the past decade.